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Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study.
Genes Nutr. 2019; 14:2.GN

Abstract

BACKGROUND

Nearly 10 years ago, the World Health Organization reported the increasing prevalence of overweight and obesity worldwide as a challenge for public health due to the associated adverse consequences. Epidemiological studies established a firm relationship between an elevated body mass index and chronic conditions such as diabetes, dyslipidemia, hypertension, heart disease, non-alcoholic fatty liver disease, and some types of cancer. Omic studies demonstrated that microRNA (miRNA) profile changes in tissues correlate with a number of diseases, including obesity. Recent studies showed a remarkable stability of miRNAs also in blood, emphasizing their potential as theranostic agents for a variety of disorders and conditions. A number of miRNAs enriched in homeostasis of obesity and metabolic disorders have been characterized in previous researches.

AIM

This work was finalized to investigate the differential circulating miRNAs signature in early childhood obesity. Our cross-sectional study analyzed the signature of circulating miRNAs in plasma samples of normal weight (n = 159) and overweight/obese (n = 149) children and adolescents participating to the I.Family study, an EC-funded study finalized to investigate the etiology of overweight, obesity and related disorders and the determinants of food choice, lifestyle, and related health outcomes in children and adolescents of eight European countries (www.ifamilystudy.eu).

RESULTS

Differences in miRNA signature with respect to anthropometric and biochemical variables were analyzed. A high degree of variability in levels of circulating miRNAs was identified among children from different countries, in line with recent reports supporting the hypothesis that these molecules are likewise affected by environmental and lifestyle factors. A panel of miRNAs differentially expressed in overweight/low-grade obesity children was characterized (miR-551a and miR-501-5p resulted upregulated; miR-10b-5p, miR-191-3p, miR-215-5p, and miR-874-3p resulted downregulated). ROC curves were also constructed for experimentally confirmed miRNAs. Single miRNAs generally exhibited low AUC values with the highest values for miR-874-3p and miR-501-5p which in combination provided an interesting value (AUC = 0.782). Pearson's analysis confirmed that miR-10b-5p, miR-215-5p, miR-501-5p, miR-551a, and miR-874-3p significantly correlated with BMI z-score. Molecular interactions of obesity-associated miRNAs were also predicted by bioinformatics tools.

CONCLUSIONS

Our work showed that several circulating miRNAs are differentially represented in overweight/low-grade obesity children and adolescents. Although causal pathways cannot be firmly inferred, it is conceivable that circulating miRNAs may be new biomarkers of early childhood obesity.

TRIAL REGISTRATION

ISRCTN, ISRCTN62310987. Registered 23/02/2018 - Retrospectively registered.

Authors+Show Affiliations

1Institute of Food Sciences, National Research Council, ISA-CNR, Via Roma, 64 83100 Avellino, Italy.1Institute of Food Sciences, National Research Council, ISA-CNR, Via Roma, 64 83100 Avellino, Italy.1Institute of Food Sciences, National Research Council, ISA-CNR, Via Roma, 64 83100 Avellino, Italy.1Institute of Food Sciences, National Research Council, ISA-CNR, Via Roma, 64 83100 Avellino, Italy.1Institute of Food Sciences, National Research Council, ISA-CNR, Via Roma, 64 83100 Avellino, Italy.2Leibniz-Institute for Prevention Research and Epidemiology, BIPS, Bremen, Germany.3University of Ghent, Ghent, Belgium.4Stazione Zoologica Anton Dohrn, Naples, Italy.2Leibniz-Institute for Prevention Research and Epidemiology, BIPS, Bremen, Germany.2Leibniz-Institute for Prevention Research and Epidemiology, BIPS, Bremen, Germany.5Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.6Medical School, University of Pécs, Pécs, Hungary.7University of Zaragoza, Zaragoza, Spain.Research and Education Institute of Child Health, Strovolos, Cyprus.9National Institute for Health Development, Tallinn, Estonia.1Institute of Food Sciences, National Research Council, ISA-CNR, Via Roma, 64 83100 Avellino, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30651891

Citation

Iacomino, Giuseppe, et al. "Circulating microRNAs Are Associated With Early Childhood Obesity: Results of the I.Family Study." Genes & Nutrition, vol. 14, 2019, p. 2.
Iacomino G, Russo P, Marena P, et al. Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study. Genes Nutr. 2019;14:2.
Iacomino, G., Russo, P., Marena, P., Lauria, F., Venezia, A., Ahrens, W., De Henauw, S., De Luca, P., Foraita, R., Günther, K., Lissner, L., Molnár, D., Moreno, L. A., Tornaritis, M., Veidebaum, T., & Siani, A. (2019). Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study. Genes & Nutrition, 14, 2. https://doi.org/10.1186/s12263-018-0622-6
Iacomino G, et al. Circulating microRNAs Are Associated With Early Childhood Obesity: Results of the I.Family Study. Genes Nutr. 2019;14:2. PubMed PMID: 30651891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study. AU - Iacomino,Giuseppe, AU - Russo,Paola, AU - Marena,Pasquale, AU - Lauria,Fabio, AU - Venezia,Antonella, AU - Ahrens,Wolfgang, AU - De Henauw,Stefaan, AU - De Luca,Pasquale, AU - Foraita,Ronja, AU - Günther,Kathrin, AU - Lissner,Lauren, AU - Molnár,Dénes, AU - Moreno,Luis A, AU - Tornaritis,Michael, AU - Veidebaum,Toomas, AU - Siani,Alfonso, Y1 - 2019/01/09/ PY - 2018/9/10/received PY - 2018/11/29/accepted PY - 2019/1/18/entrez PY - 2019/1/18/pubmed PY - 2019/1/18/medline KW - Biomarker KW - Childhood obesity KW - Childhood overweight/low-grade obesity KW - Circulating miRNAs KW - Metabolic disorders SP - 2 EP - 2 JF - Genes & nutrition JO - Genes Nutr VL - 14 N2 - BACKGROUND: Nearly 10 years ago, the World Health Organization reported the increasing prevalence of overweight and obesity worldwide as a challenge for public health due to the associated adverse consequences. Epidemiological studies established a firm relationship between an elevated body mass index and chronic conditions such as diabetes, dyslipidemia, hypertension, heart disease, non-alcoholic fatty liver disease, and some types of cancer. Omic studies demonstrated that microRNA (miRNA) profile changes in tissues correlate with a number of diseases, including obesity. Recent studies showed a remarkable stability of miRNAs also in blood, emphasizing their potential as theranostic agents for a variety of disorders and conditions. A number of miRNAs enriched in homeostasis of obesity and metabolic disorders have been characterized in previous researches. AIM: This work was finalized to investigate the differential circulating miRNAs signature in early childhood obesity. Our cross-sectional study analyzed the signature of circulating miRNAs in plasma samples of normal weight (n = 159) and overweight/obese (n = 149) children and adolescents participating to the I.Family study, an EC-funded study finalized to investigate the etiology of overweight, obesity and related disorders and the determinants of food choice, lifestyle, and related health outcomes in children and adolescents of eight European countries (www.ifamilystudy.eu). RESULTS: Differences in miRNA signature with respect to anthropometric and biochemical variables were analyzed. A high degree of variability in levels of circulating miRNAs was identified among children from different countries, in line with recent reports supporting the hypothesis that these molecules are likewise affected by environmental and lifestyle factors. A panel of miRNAs differentially expressed in overweight/low-grade obesity children was characterized (miR-551a and miR-501-5p resulted upregulated; miR-10b-5p, miR-191-3p, miR-215-5p, and miR-874-3p resulted downregulated). ROC curves were also constructed for experimentally confirmed miRNAs. Single miRNAs generally exhibited low AUC values with the highest values for miR-874-3p and miR-501-5p which in combination provided an interesting value (AUC = 0.782). Pearson's analysis confirmed that miR-10b-5p, miR-215-5p, miR-501-5p, miR-551a, and miR-874-3p significantly correlated with BMI z-score. Molecular interactions of obesity-associated miRNAs were also predicted by bioinformatics tools. CONCLUSIONS: Our work showed that several circulating miRNAs are differentially represented in overweight/low-grade obesity children and adolescents. Although causal pathways cannot be firmly inferred, it is conceivable that circulating miRNAs may be new biomarkers of early childhood obesity. TRIAL REGISTRATION: ISRCTN, ISRCTN62310987. Registered 23/02/2018 - Retrospectively registered. SN - 1555-8932 UR - https://cancerres.unboundmedicine.com/medline/citation/30651891/Circulating_microRNAs_are_associated_with_early_childhood_obesity:_results_of_the_I_Family_Study_ DB - PRIME DP - Unbound Medicine ER -