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Changes in circulating miRNAs in healthy overweight and obese subjects: Effect of diet composition and weight loss.
Clin Nutr. 2019 02; 38(1):438-443.CN

Abstract

BACKGROUND

MicroRNAs (miRNAs) are small non-coding RNA molecules that can play an important role in several chronic metabolic conditions, including obesity. However, to date little is known about how they are regulated. Weight loss induced by surgical procedures has been effective at modulating specific circulating miRNAs, but the effect of energy-restricted diets with different macronutrient compositions on circulating miRNAs is not well understood. The objective of the present analysis was to explore the effect of three energy-restricted diets of different macronutrient composition and carbohydrate quality on plasma miRNA levels.

METHODS

The GLYNDIET study is a 6-month, parallel, randomized clinical trial conducted on overweight and obese subjects who were randomized to one of three different dietary intervention groups: i) a moderate-carbohydrate and low glycemic index diet (LGI), ii) a moderate-carbohydrate and high glycemic index diet (HGI), and iii) a low-fat and high glycemic index diet (LF). We assessed the genome-wide circulating miRNA profile in a subsample of eight randomly selected participants. A total of 8 miRNAs (miR-411, miR-432, miR-99b, miR-340, miR-423, miR-361, let-7c) were differently quantified according to diet intervention, and were therefore longitudinally validated in 103 participants before and after the energy-restricted diets.

RESULTS

Circulating miR-361 levels were lower in the LGI group than in the HGI group, even after adjusting for differences in weight loss. The intra-group analyses demonstrated a significant down-regulation of all miRNAs screened in our study subjects after the LGI intervention. Similarly, miR-139 and miR-340 were down-regulated after the HGI intervention, while miR-139, miR-432 and miR-423 were down-regulated after the low-fat diet. Changes in circulating miR-139 and let-7c were significantly associated with changes in lipid profile and insulin resistance.

CONCLUSION

An energy-restricted low-glycemic index diet down-regulates circulating miRNA-361 more than an energy-restricted high-glycemic index, regardless of the magnitude of the weight loss.

Authors+Show Affiliations

Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. Electronic address: jordi.salas@urv.cat.Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. Electronic address: monica.bullo@urv.cat.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29233588

Citation

Giardina, S, et al. "Changes in Circulating miRNAs in Healthy Overweight and Obese Subjects: Effect of Diet Composition and Weight Loss." Clinical Nutrition (Edinburgh, Scotland), vol. 38, no. 1, 2019, pp. 438-443.
Giardina S, Hernández-Alonso P, Díaz-López A, et al. Changes in circulating miRNAs in healthy overweight and obese subjects: Effect of diet composition and weight loss. Clin Nutr. 2019;38(1):438-443.
Giardina, S., Hernández-Alonso, P., Díaz-López, A., Salas-Huetos, A., Salas-Salvadó, J., & Bulló, M. (2019). Changes in circulating miRNAs in healthy overweight and obese subjects: Effect of diet composition and weight loss. Clinical Nutrition (Edinburgh, Scotland), 38(1), 438-443. https://doi.org/10.1016/j.clnu.2017.11.014
Giardina S, et al. Changes in Circulating miRNAs in Healthy Overweight and Obese Subjects: Effect of Diet Composition and Weight Loss. Clin Nutr. 2019;38(1):438-443. PubMed PMID: 29233588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in circulating miRNAs in healthy overweight and obese subjects: Effect of diet composition and weight loss. AU - Giardina,S, AU - Hernández-Alonso,P, AU - Díaz-López,A, AU - Salas-Huetos,A, AU - Salas-Salvadó,J, AU - Bulló,M, Y1 - 2017/12/01/ PY - 2017/06/06/received PY - 2017/10/20/revised PY - 2017/11/19/accepted PY - 2017/12/14/pubmed PY - 2020/3/26/medline PY - 2017/12/14/entrez KW - Diet KW - MicroRNAs KW - Weight loss SP - 438 EP - 443 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 38 IS - 1 N2 - BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNA molecules that can play an important role in several chronic metabolic conditions, including obesity. However, to date little is known about how they are regulated. Weight loss induced by surgical procedures has been effective at modulating specific circulating miRNAs, but the effect of energy-restricted diets with different macronutrient compositions on circulating miRNAs is not well understood. The objective of the present analysis was to explore the effect of three energy-restricted diets of different macronutrient composition and carbohydrate quality on plasma miRNA levels. METHODS: The GLYNDIET study is a 6-month, parallel, randomized clinical trial conducted on overweight and obese subjects who were randomized to one of three different dietary intervention groups: i) a moderate-carbohydrate and low glycemic index diet (LGI), ii) a moderate-carbohydrate and high glycemic index diet (HGI), and iii) a low-fat and high glycemic index diet (LF). We assessed the genome-wide circulating miRNA profile in a subsample of eight randomly selected participants. A total of 8 miRNAs (miR-411, miR-432, miR-99b, miR-340, miR-423, miR-361, let-7c) were differently quantified according to diet intervention, and were therefore longitudinally validated in 103 participants before and after the energy-restricted diets. RESULTS: Circulating miR-361 levels were lower in the LGI group than in the HGI group, even after adjusting for differences in weight loss. The intra-group analyses demonstrated a significant down-regulation of all miRNAs screened in our study subjects after the LGI intervention. Similarly, miR-139 and miR-340 were down-regulated after the HGI intervention, while miR-139, miR-432 and miR-423 were down-regulated after the low-fat diet. Changes in circulating miR-139 and let-7c were significantly associated with changes in lipid profile and insulin resistance. CONCLUSION: An energy-restricted low-glycemic index diet down-regulates circulating miRNA-361 more than an energy-restricted high-glycemic index, regardless of the magnitude of the weight loss. SN - 1532-1983 UR - https://cancerres.unboundmedicine.com/medline/citation/29233588/Changes_in_circulating_miRNAs_in_healthy_overweight_and_obese_subjects:_Effect_of_diet_composition_and_weight_loss_ DB - PRIME DP - Unbound Medicine ER -