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Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers.
Pediatr Obes. 2017 06; 12(3):229-238.PO

Abstract

BACKGROUND

Circulating microRNAs (miRNAs) may act as biomarkers of metabolic disturbances.

OBJECTIVE

The aim of this study was to identify serum miRNAs signature of early insulin resistance in obese preschoolers.

METHODS

Twelve obese children, aged 2-6 years, six insulin resistant (IR) and six controls were selected being age-matched, sex-matched and body mass index-matched. Profiling of 179 circulating miRNAs, known to be widely expressed in the bloodstream, was investigated by quantitative polymerase chain reaction at fasting and 120 min following a standard oral glucose tolerance test (OGTT).

RESULTS

Twenty-one miRNAs were differentially regulated in IR obese preschoolers. miR-200c-3p, miR-190a and miR-95 were differently regulated both at fasting and 120 min after the OGTT. In controls, the fold changes of some miRNAs were correlated with Δglucose0-120 (miR-660, miR-26b-5p and miR-22-3p: p = 0.005 for all) and Δinsulin0-120 (miR-660 and miR-22-3p: p = 0.02 for both and miR-423-5p: p = 0.042). In IR patients, miR-1 fold changes were correlated with Δglucose0-120(r = -0.786; p = 0.036) and Δinsulin0-120(r = -0.821; p = 0.023).

CONCLUSIONS

Our study identifies circulating miR-200c-3p, miR-190a and miR-95 as biomarkers of insulin resistance in obese preschoolers, being differentially regulated in IR patients both in fasting condition and after the OGTT. Expression of some circulating miRNAs seems reflecting glucose and insulin excursion following the OGTT differently in controls and IR obese preschoolers.

Authors+Show Affiliations

Gene expression - Microarrays Laboratory, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.Gene expression - Microarrays Laboratory, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.Research Unit for Multifactorial Disease, Scientific Directorate, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.Federazione Italiana Medici Pediatri, FIMP, Rome, Italy.Federazione Italiana Medici Pediatri, FIMP, Rome, Italy.Federazione Italiana Medici Pediatri, FIMP, Rome, Italy.Federazione Italiana Medici Pediatri, FIMP, Rome, Italy.Federazione Italiana Medici Pediatri, FIMP, Rome, Italy.Research Unit for Multifactorial Disease, Scientific Directorate, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27059430

Citation

Masotti, A, et al. "Oral Glucose Tolerance Test Unravels Circulating miRNAs Associated With Insulin Resistance in Obese Preschoolers." Pediatric Obesity, vol. 12, no. 3, 2017, pp. 229-238.
Masotti A, Baldassarre A, Fabrizi M, et al. Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers. Pediatr Obes. 2017;12(3):229-238.
Masotti, A., Baldassarre, A., Fabrizi, M., Olivero, G., Loreti, M. C., Giammaria, P., Veronelli, P., Graziani, M. P., & Manco, M. (2017). Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers. Pediatric Obesity, 12(3), 229-238. https://doi.org/10.1111/ijpo.12133
Masotti A, et al. Oral Glucose Tolerance Test Unravels Circulating miRNAs Associated With Insulin Resistance in Obese Preschoolers. Pediatr Obes. 2017;12(3):229-238. PubMed PMID: 27059430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers. AU - Masotti,A, AU - Baldassarre,A, AU - Fabrizi,M, AU - Olivero,G, AU - Loreti,M C, AU - Giammaria,P, AU - Veronelli,P, AU - Graziani,M P, AU - Manco,M, Y1 - 2016/04/05/ PY - 2015/07/24/received PY - 2016/02/22/revised PY - 2016/02/26/accepted PY - 2016/4/10/pubmed PY - 2017/8/5/medline PY - 2016/4/10/entrez KW - Insulin resistance KW - OGTT KW - microRNAs KW - obesity KW - preschooler SP - 229 EP - 238 JF - Pediatric obesity JO - Pediatr Obes VL - 12 IS - 3 N2 - BACKGROUND: Circulating microRNAs (miRNAs) may act as biomarkers of metabolic disturbances. OBJECTIVE: The aim of this study was to identify serum miRNAs signature of early insulin resistance in obese preschoolers. METHODS: Twelve obese children, aged 2-6 years, six insulin resistant (IR) and six controls were selected being age-matched, sex-matched and body mass index-matched. Profiling of 179 circulating miRNAs, known to be widely expressed in the bloodstream, was investigated by quantitative polymerase chain reaction at fasting and 120 min following a standard oral glucose tolerance test (OGTT). RESULTS: Twenty-one miRNAs were differentially regulated in IR obese preschoolers. miR-200c-3p, miR-190a and miR-95 were differently regulated both at fasting and 120 min after the OGTT. In controls, the fold changes of some miRNAs were correlated with Δglucose0-120 (miR-660, miR-26b-5p and miR-22-3p: p = 0.005 for all) and Δinsulin0-120 (miR-660 and miR-22-3p: p = 0.02 for both and miR-423-5p: p = 0.042). In IR patients, miR-1 fold changes were correlated with Δglucose0-120(r = -0.786; p = 0.036) and Δinsulin0-120(r = -0.821; p = 0.023). CONCLUSIONS: Our study identifies circulating miR-200c-3p, miR-190a and miR-95 as biomarkers of insulin resistance in obese preschoolers, being differentially regulated in IR patients both in fasting condition and after the OGTT. Expression of some circulating miRNAs seems reflecting glucose and insulin excursion following the OGTT differently in controls and IR obese preschoolers. SN - 2047-6310 UR - https://cancerres.unboundmedicine.com/medline/citation/27059430/Oral_glucose_tolerance_test_unravels_circulating_miRNAs_associated_with_insulin_resistance_in_obese_preschoolers_ DB - PRIME DP - Unbound Medicine ER -