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Changes in circulating microRNAs are associated with childhood obesity.
J Clin Endocrinol Metab. 2013 Oct; 98(10):E1655-60.JC

Abstract

CONTEXT

Circulating microRNAs (miRNAs) are valuable biomarkers of metabolic diseases and potential therapeutic targets in this field.

OBJECTIVE

Our objective was to define the circulating pattern of miRNAs in childhood obesity. DESIGN, SETTINGS, AND MAIN OUTCOME MEASURE: The genome-wide circulating miRNA profile was assessed by RT-PCR in 10 boys (5 lean and 5 obese children). The most relevant miRNAs were cross-sectionally validated in 85 lean versus 40 obese children (63 boys and 62 girls) and longitudinally evaluated in samples from the same children when they were ≈ 7 and ≈ 10 years old (23 boys and 22 girls).

RESULTS

The cross-sectional validation study disclosed that 15 specific circulating miRNAs were significantly deregulated in prepubertal obesity, including the decreased miR-221 and miR-28-3p and increased concentrations in plasma of miR-486-5p, miR-486-3p, miR-142-3p, miR-130b, and miR-423-5p (all P < .0001). The circulating concentration of these miRNAs was significantly associated with body mass index and other measures of obesity such as percent fat mass, waist, regional fat distribution and with laboratory parameters such as homeostasis model assessment of insulin resistance, high-molecular-weight adiponectin, C-reactive protein, and circulating lipids in concordance with anthropometric associations. Plasma concentrations of 10 of these circulating miRNAs changed significantly and differently during the 3-year follow-up in children who increased or decreased their normalized weight.

CONCLUSION

This study provides the first evidence that circulating miRNAs are deregulated in prepubertal obese children. Thus, the very early detection of an abnormal circulating miRNA profile may be a promising strategy to identify obese children who may suffer from metabolic abnormalities.

Authors+Show Affiliations

MD, PhD, Section of Diabetes, Endocrinology, and Nutrition. Hospital "Dr Josep Trueta" of Girona, Carretera de França s/n, 17007 Girona, Spain. jmfreal@idibgi.org.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23928666

Citation

Prats-Puig, Anna, et al. "Changes in Circulating microRNAs Are Associated With Childhood Obesity." The Journal of Clinical Endocrinology and Metabolism, vol. 98, no. 10, 2013, pp. E1655-60.
Prats-Puig A, Ortega FJ, Mercader JM, et al. Changes in circulating microRNAs are associated with childhood obesity. J Clin Endocrinol Metab. 2013;98(10):E1655-60.
Prats-Puig, A., Ortega, F. J., Mercader, J. M., Moreno-Navarrete, J. M., Moreno, M., Bonet, N., Ricart, W., López-Bermejo, A., & Fernández-Real, J. M. (2013). Changes in circulating microRNAs are associated with childhood obesity. The Journal of Clinical Endocrinology and Metabolism, 98(10), E1655-60. https://doi.org/10.1210/jc.2013-1496
Prats-Puig A, et al. Changes in Circulating microRNAs Are Associated With Childhood Obesity. J Clin Endocrinol Metab. 2013;98(10):E1655-60. PubMed PMID: 23928666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in circulating microRNAs are associated with childhood obesity. AU - Prats-Puig,Anna, AU - Ortega,Francisco J, AU - Mercader,Josep M, AU - Moreno-Navarrete,José M, AU - Moreno,María, AU - Bonet,Nuria, AU - Ricart,Wifredo, AU - López-Bermejo,Abel, AU - Fernández-Real,José M, Y1 - 2013/08/08/ PY - 2013/8/10/entrez PY - 2013/8/10/pubmed PY - 2013/12/16/medline SP - E1655 EP - 60 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 98 IS - 10 N2 - CONTEXT: Circulating microRNAs (miRNAs) are valuable biomarkers of metabolic diseases and potential therapeutic targets in this field. OBJECTIVE: Our objective was to define the circulating pattern of miRNAs in childhood obesity. DESIGN, SETTINGS, AND MAIN OUTCOME MEASURE: The genome-wide circulating miRNA profile was assessed by RT-PCR in 10 boys (5 lean and 5 obese children). The most relevant miRNAs were cross-sectionally validated in 85 lean versus 40 obese children (63 boys and 62 girls) and longitudinally evaluated in samples from the same children when they were ≈ 7 and ≈ 10 years old (23 boys and 22 girls). RESULTS: The cross-sectional validation study disclosed that 15 specific circulating miRNAs were significantly deregulated in prepubertal obesity, including the decreased miR-221 and miR-28-3p and increased concentrations in plasma of miR-486-5p, miR-486-3p, miR-142-3p, miR-130b, and miR-423-5p (all P < .0001). The circulating concentration of these miRNAs was significantly associated with body mass index and other measures of obesity such as percent fat mass, waist, regional fat distribution and with laboratory parameters such as homeostasis model assessment of insulin resistance, high-molecular-weight adiponectin, C-reactive protein, and circulating lipids in concordance with anthropometric associations. Plasma concentrations of 10 of these circulating miRNAs changed significantly and differently during the 3-year follow-up in children who increased or decreased their normalized weight. CONCLUSION: This study provides the first evidence that circulating miRNAs are deregulated in prepubertal obese children. Thus, the very early detection of an abnormal circulating miRNA profile may be a promising strategy to identify obese children who may suffer from metabolic abnormalities. SN - 1945-7197 UR - https://cancerres.unboundmedicine.com/medline/citation/23928666/Changes_in_circulating_microRNAs_are_associated_with_childhood_obesity_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2013-1496 DB - PRIME DP - Unbound Medicine ER -