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Alternative evaluation method in vitro for the water-resistant effect of sunscreen products.
Skin Res Technol. 2008 May; 14(2):187-91.SR

Abstract

BACKGROUND/PURPOSE

Sunscreen products today represent a trend of providing not only simple sun protection factor (SPF)/protection of UVA (PFA) but also other additional benefits. For example, as popularized by seasonless use of sunscreens, the special function of water resistance or sand proof is added to sunscreens as well as for leisure. Because a human in vivo test is time consuming and expensive, a screening process has been tried using an accurate in vitro system. In this study, we suggest the development of an in vitro test that can predict the result of in vivo water resistance of sunscreens.

METHODS

Water resistance is presented as a comparison of initial SPF and water-exposed SPF by immersion and washing. In order to be comparable with the in vivo test, water immersion and flow were defined as the basic statements. Also, substrate, revolutions per minute (r.p.m.)--rotative velocity--of propeller inducing water flow, and time of immersion were defined as controlled factors. Considering the strength, separation of test material and adhesive texture, a PMMA plate was selected as suitable among commercial substrates: Transpore tape, VITRO SKIN, and PMMA plate. Also, when the PMMA plate was adhered on the wall of a water bath, the water turbulence of the rotational propeller alone was not strong enough to wash off the test material from the substrate. Therefore, PMMA plates were fixed on the axis. In this experiment, the most important thing is whether this in vitro system can predict correctly. Hence, we tried to match the in vitro water resistance following from our control factors and water resistance value of the in vivo test.

RESULTS

We found the immersion time and r.p.m. of controlled factors to obtain the target water resistance using design of experiment, MiniTab statistical package. Response optimization yielded the optimal in vitro conditions of 150 r.p.m./60 min. The repeatability and reproducibility of this in vitro system were also good in validation studies.

CONCLUSIONS

This study enables to modify an in vivo water resistance test and predict the result of in vivo water resistance by the manufacture of effective equipment and choosing a suitable substrate. Compared with in vivo results, our in vitro system is more time and cost effective, and provides reliable results.

Authors+Show Affiliations

Skin Research Institute, Amore-Pacific Corporation, Yongin, Korea. syahn@amorepacific.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

18412561

Citation

Ahn, Sungyeon, et al. "Alternative Evaluation Method in Vitro for the Water-resistant Effect of Sunscreen Products." Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI), vol. 14, no. 2, 2008, pp. 187-91.
Ahn S, Yang H, Lee H, et al. Alternative evaluation method in vitro for the water-resistant effect of sunscreen products. Skin Res Technol. 2008;14(2):187-91.
Ahn, S., Yang, H., Lee, H., Moon, S., & Chang, I. (2008). Alternative evaluation method in vitro for the water-resistant effect of sunscreen products. Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI), 14(2), 187-91. https://doi.org/10.1111/j.1600-0846.2007.00276.x
Ahn S, et al. Alternative Evaluation Method in Vitro for the Water-resistant Effect of Sunscreen Products. Skin Res Technol. 2008;14(2):187-91. PubMed PMID: 18412561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alternative evaluation method in vitro for the water-resistant effect of sunscreen products. AU - Ahn,Sungyeon, AU - Yang,Hyejin, AU - Lee,Haekwang, AU - Moon,Seongjoon, AU - Chang,Ihseop, PY - 2008/4/17/pubmed PY - 2008/5/7/medline PY - 2008/4/17/entrez SP - 187 EP - 91 JF - Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) JO - Skin Res Technol VL - 14 IS - 2 N2 - BACKGROUND/PURPOSE: Sunscreen products today represent a trend of providing not only simple sun protection factor (SPF)/protection of UVA (PFA) but also other additional benefits. For example, as popularized by seasonless use of sunscreens, the special function of water resistance or sand proof is added to sunscreens as well as for leisure. Because a human in vivo test is time consuming and expensive, a screening process has been tried using an accurate in vitro system. In this study, we suggest the development of an in vitro test that can predict the result of in vivo water resistance of sunscreens. METHODS: Water resistance is presented as a comparison of initial SPF and water-exposed SPF by immersion and washing. In order to be comparable with the in vivo test, water immersion and flow were defined as the basic statements. Also, substrate, revolutions per minute (r.p.m.)--rotative velocity--of propeller inducing water flow, and time of immersion were defined as controlled factors. Considering the strength, separation of test material and adhesive texture, a PMMA plate was selected as suitable among commercial substrates: Transpore tape, VITRO SKIN, and PMMA plate. Also, when the PMMA plate was adhered on the wall of a water bath, the water turbulence of the rotational propeller alone was not strong enough to wash off the test material from the substrate. Therefore, PMMA plates were fixed on the axis. In this experiment, the most important thing is whether this in vitro system can predict correctly. Hence, we tried to match the in vitro water resistance following from our control factors and water resistance value of the in vivo test. RESULTS: We found the immersion time and r.p.m. of controlled factors to obtain the target water resistance using design of experiment, MiniTab statistical package. Response optimization yielded the optimal in vitro conditions of 150 r.p.m./60 min. The repeatability and reproducibility of this in vitro system were also good in validation studies. CONCLUSIONS: This study enables to modify an in vivo water resistance test and predict the result of in vivo water resistance by the manufacture of effective equipment and choosing a suitable substrate. Compared with in vivo results, our in vitro system is more time and cost effective, and provides reliable results. SN - 1600-0846 UR - https://cancerres.unboundmedicine.com/medline/citation/18412561/Alternative_evaluation_method_in_vitro_for_the_water_resistant_effect_of_sunscreen_products_ DB - PRIME DP - Unbound Medicine ER -